FIGURE 6. Immunohistochemical mapping of GABAR α1, α6, β2, β3, γ2, and δ subunits in the cerebellum of control (+/+) and stargazer (stg) mice.

Adult +/+ and stg mice were perfusion-fixed with paraformaldehyde, cryostat-sectioned, and immunostained as described under “Experimental Procedures.” Anti-GABAR subunit-specific antibodies were optimized for signal:noise ratio. Final concentrations used were α1 (0.5 μg/ml), α6 (0.06 μg/ml), β2 (0.5 μg/ml), β3 (0.5 μg/ml), γ2 (0.5 μg/ml), and δ (0.25 μg/ml). Each panel shows a low power image of an immunostained whole cerebellar section and a high power image cross-section of a typical cerebellar lobule from each mouse strain. These are representative results derived from a minimum of three +/+ and three stg brains. ML, molecular layer; GL, granular layer.