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. Author manuscript; available in PMC: 2010 Nov 5.
Published in final edited form as: J Biol Chem. 2007 Jul 23;282(40):29130–29143. doi: 10.1074/jbc.M700111200

FIGURE 8. Postembedding immunocytochemistry on electron microscopic sections.

FIGURE 8

A, immunolabeling of GABAR α6 subunit in the cerebellar granule cell layer of +/+ (panels A, B, D, and E) or stg (panels C and F) mouse cerebellum. The plasma membrane of granule cell bodies from +/+ mice (panels A and B) are labeled by gold particles (arrows), but the plasma membrane of stg mice (panel C; arrowheads) are not labeled, and the arrow in panel C marks a gold particle representing nonspecific-labeling of a mitochondrial profile. Plasma membrane of a dendrite (containing mitochondrial profiles) from +/+ mice is strongly labeled but those from stg mice are not labeled (arrow in panel F represents background labeling over a mitochondrial profile). Bars represent 500 nm in A and 250 nm in panels B–F. Two +/+ and two stg brains were fixed by each perfusion method. B, quantitative analysis of mean labeling density (number of gold particles/unit membrane length) of GABAR α6 immunoreactivity in various extrasynaptic (soma and dendrites) and synaptic subdomains of cerebellar granule cells from +/+ (closed boxes) and stg mice (open boxes) in situ. Data are representative of the mean ± S.E.