TABLE 1. Quantification of GABAR subunit proteins by Western blot analysis of cerebellar membranes derived from adult stargazer (stg) mouse cerebella relative to age-matched controls (+/+).

Equal amounts of cerebellar membrane protein from age-matched adult +/+ and stg mice were separated by SDS-PAGE and subjected to Western blot analysis, and immunoreactive band intensities were determined by ImageJ analysis as described under “Experimental Procedures.” Results were obtained from three to six +/+ and three to six stg mice that were investigated a total of three to five times and are expressed as percentages of the weighted average subunit level found in +/+ mice ± S.E. For statistical comparison unpaired Student’s t test was used. n indicates number of individual animals tested; NS indicates not significant.

Protein	+/+ = 100% (mean ± S.E.)	n	stg % of +/+ (mean ± S.E.)	n	Significance (p value)
α1	100 ± 2	3	85 ± 1	3	<0.001
α6	100 ± 3	6	49 ± 2	6	<0.0001
β2	100 ± 3	3	85 ± 2	3	<0.01
β3	100 ± 3	3	64 ± 5	3	<0.001
γ2	100 ± 2	3	95 ± 4	3	NS
δ	100 ± 2	6	52 ± 3	6	<0.0001