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Analysis of p53 activity in yeast utilizing a weak p53 binding promoter element. Yeast strain yIG397 transformed with pRDI-22 (vector), pLS76 (WTp53), or plasmids expressing one of the p53 mutants indicated, generated via gap repair, were pregrown on high-adenine-containing solid media and then streaked on low-adenine-containing solid media to score for adenine auxotrophy.
