Table II.

PEGylation Optimization of BSA Nanoparticles Using Central Composite Design

Trial	Coded Factors				Experimental Results
	X 1	X 2	X 3	X 4	Molar Ratio of PEGylated Amino Groups per bare BSA Nanoparticles	PEGylation Efficiency (%)
1	−1	−1	−1	−1	2.77025	35
2	1	−1	−1	−1	4.2959	54
3	−1	1	−1	−1	4.62282	59
4	1	1	−1	−1	4.96897	63
5	−1	−1	1	−1	6.469	82
6	1	−1	1	−1	7.7767	97
7	−1	1	1	−1	5.6292	71
8	1	1	1	−1	7.43605	94
9	−1	−1	−1	1	2.6741	34
10	1	−1	−1	1	5.7254	73
11	−1	1	−1	1	5.5587	71
12	1	1	−1	1	7.50743	95
13	−1	−1	1	1	3.45615	44
14	1	−1	1	1	7.59077	96
15	−1	1	1	1	6.84077	87
16	1	1	1	1	7.83436	98
17	−2	0	0	0	3.09718	39
18	2	0	0	0	7.64846	95
19	0	−2	0	0	4.63564	59
20	0	2	0	0	7.78308	97
21	0	0	−2	0	5.8664	74
22	0	0	2	0	7.53948	95
23	0	0	0	−2	5.7446	73
24	0	0	0	2	6.6613	85
25	0	0	0	0	7.10359	90
26	0	0	0	0	7.10361	90
27	0	0	0	0	7.1036	90
28	0	0	0	0	7.10359	90
29	0	0	0	0	7.10358	90
30	0	0	0	0	7.10362	90
31	0	0	0	0	7.10359	90