Table II.
Formulation of Cyt-PLGA NP for Optimization of Volume of Co-solvent and Non-solvent
| Batch No. | Real value | Transformed values | Response | |||||
|---|---|---|---|---|---|---|---|---|
| Volume of the co-solvent (ml) X1 | Volume of the non-solvent (ml) X2 | X1 | X2 | X12 | X22 | X1X2 | MPS (nm) ± SDa Y1 | |
| CPNP4 | 0.3 | 2 | −1 | −1 | 1 | 1 | 1 | 147 ± 7.1 |
| CPNP5 | 0.3 | 5 | −1 | 0 | 1 | 0 | 0 | 137 ± 2.3 |
| CPNP6 | 0.3 | 8 | −1 | 1 | 1 | 1 | −1 | 142 ± 6.2 |
| CPNP7 | 0.6 | 2 | 0 | −1 | 0 | 1 | 0 | 137 ± 7.6 |
| CPNP8 | 0.6 | 5 | 0 | 0 | 0 | 0 | 0 | 127 ± 3.1 |
| CPNP9 | 0.6 | 8 | 0 | 1 | 0 | 1 | 0 | 134 ± 5.2 |
| CPNP10 | 0.9 | 2 | 1 | −1 | 1 | 1 | −1 | 141 ± 3.4 |
| CPNP11 | 0.9 | 5 | 1 | 0 | 1 | 0 | 0 | 137 ± 2.5 |
| CPNP12 | 0.9 | 8 | 1 | 1 | 1 | 1 | 1 | 148 ± 5.6 |
Batches taken as per 32 factorial design: factors, their levels, transformed values and response: MPS
MPS mean particle size
aStandard deviation (n = 3)