Table I.

Validation Parameters for Estradiol Analysis by HPLC

Validation parameter	Value
Regression equation (n = 7)
Linear range (μg.mL−1)	0.250–8.00
Limit of detectiona (μg.mL−1)	0.08
Limit of quantitationb (μg.mL−1)	0.24
Precision (n = 7)
Repeatability
SD of intra-assay	0.136–2.42
RSD of intra-assay	0.408–1.14
Intermediate precision
SD of inter days	0.375–5.78
RSD of inter days	0.578–3.21
Accuracy (n = 7)
RE%c	0.156–8.87
Recovery%	91.1–101.3

^aSignal to noise ratio (SNR) > 3

^bSignal to noise ratio (SNR) > 10

^cRelative error percentage