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. Author manuscript; available in PMC: 2010 Nov 5.
Published in final edited form as: J Immunol Methods. 2009 Sep 2;350(1-2):171–182. doi: 10.1016/j.jim.2009.08.013

Figure 1. Schematic of the bead ELISA for the detection of antibodies in serum.

Figure 1

The cDNAs encoding full-length antigens are individually expressed as GST-tagged fusion proteins using rabbit reticulocyte lysate. Recombinant proteins are captured onto anti-GST-coupled Luminex microspheres. The beads displaying different antigens are pooled and re-aliquoted prior to addition of human serum so that there are 5000 of each color/antigen bead per serum. Human IgG is detected with PE-conjugated secondary anti-IgG antibodies.