Figure 6. Viral load and specific antibodies after Influenza A/WSN/33 H1N1 infections of WT and PAFR-deficient mice.

WT and PAFR KO mice were infected intranasally with 10⁶ PFU (n = 4–6) and killed 5 days post infection (A) or infected with 10⁴ PFU (n = 4–6) and killed 5 (B) and 8 days post infection (C). Viral titers in lungs homogenates, as assessed by MDCK plaque formation. WT and PAFR KO mice (n = 4–6) were infected intranasally with 10³ PFU and after 14 days were reinfected with 10⁶ PFU. After 21 days mice were killed and anti-Influenza A/WSN/33 H1N1 IgG titer was measured in serum samples by ELISA (D). Data are presented as Mean ± SEM. ** for p<0.01 when compared to WT infected group (unpaired t test).