Table 1.
In-111 labeled target cells, antibodies used at 5 ug/ml, human PBMC used as effector cells, 4 hour incubation at 370C before harvest.
| Target | Effector:Target Ratio | % Specific ADCC Activity (± SEM) | ||
|---|---|---|---|---|
| mAb 31.1chi | CA 19-9 | UPC-10 negative cntl | ||
| SW1463 (colorectal adeno) | 100:1 | 51.0 ± 1.3 | 2.0 ± 1.2 | 3.4 ± 0.8 |
| 50:1 | 36.0 ± 1.0 | 1.9 ± 0.1 | 1.1 ± 0.8 | |
| 25:1 | 24.4 ± 1.7 | 1.6 ± 0.3 | 1.3 ± 0.2 | |
| AsPC-1 (pancreas adeno) | 100:1 | 30.3 ± 1.3 | 19.5 ± 0.4 | 3.2 ± 0.9 |
| 50:1 | 19.3 ± 1.3 | 1.4 ± 0.4 | 0.6 ± 0.5 | |
| 25:1 | 12.7 ± 0.1 | 3.3 ± 1.1 | -0.6 ± 1.9 | |
| H441 (lung epithelial ) | 100:1 | 5.0 ±0.4 | 0.6 ± 3.0 | 3.0 ±1.2 |
| 50:1 | 5.2 ± 3.6 | 1.8 ± 0.6 | -0.5 ± 0.4 | |
| 25:1 | 6.7 ± 0.6 | 1.0 ± 0.2 | 0.1 ± 0.5 | |
| MCF-7 (breast adeno) | 100:1 | 2.4 ± 0.3 | 2.5 ± 0.5 | 2.6 ± 0.8 |
| 50:1 | 2.4 ± 0.5 | 1.7 ± 0.1 | 1.8 ± 0.3 | |
| 25:1 | 2.8 ± 0.4 | 2.5 ± 0.2 | 1.7 ± 0.1 | |