Figure 3.

Specificity of binding to cytosolic proteins in normal and regenerating male rat liver cytosol. Two-hundred microliter aliquots of male rat liver cytosol were precipitated with protamine sulfate and were incubated with 1.5 nM [³H]E₂ in the presence and absence of 100-fold excess of competing ligand for 18 h at 0°C. Each bar represents the mean results of triplicate determinations for each competing ligand. The solid bars represent data obtained using normal liver; the hatched bars represent data obtained using regenerating liver. Progesterone (PROG), testosterone (TEST), cortisone (CORT).