Figure 7.

In situ analysis of γ-irradiation-induced apoptosis of splenocytes. Spleen sections from untreated (top panels) or 5 Gy γ-irradiated (20 h earlier, bottom panels) wt (a–b), noxa^−/− (c–d), puma^−/− (e–f), noxa^−/−puma^−/− (g–h) or p53^−/− (l–j) animals were TUNEL-stained (see Figure 5). Nuclei were counterstained with hematoxylin. Sections from puma^−/− and noxa^−/−puma^−/− animals (f, h) contain very few TUNEL-positive cells, comparable with sections from p53^−/− animals (j). The images represent three or more independent stains performed on organs of at least three animals per genotype per treatment