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. 2010 Oct 15;132(44):15522–15524. doi: 10.1021/ja107677q

Figure 1.

Figure 1

(a) Overview of IDPCR. (b) IDPCR with streptavidin as the target (1a-SA) and biotin (2a-biotin, Kd = 40 pM) or desthiobiotin (2b-desthiobiotin, Kd = 2 nM) as ligands was analyzed by qPCR and PAGE (21 cycles of PCR). IDPCR reports the interaction of (c) trypsin and antipain (Ki = 100 nM); (d) carbonic anhydrase and carboxy benzene sulfonamide (CBS, Ki = 3.2 μM) or Gly-Leu-CBS (GLCBS, Ki = 9 nM); and (e) a DNA aptamer and daunomycin (Dn) (Dn, Kd = 272 nM) or doxorubicin (Dx). PAGE gels in (c), (d), and (e) show DNA after 20, 24, and 23 cycles of PCR, respectively.