. 2010 Oct;5(10):1805–1814. doi: 10.2215/CJN.01500210

Table 1.

Primer sequences and PCR conditions for amplification of MMP and TIMP SNPs

SNP	rs Number	Method	Primer	Sequence (5′ to 3′)
TIMP-1 372 C/T	rs 4898	RFLP-PCR	Forward	`GCACATCACTACCTGCAGTC`
TIMP-1 372 C/T	rs 4898	RFLP-PCR	Reverse	`GAAACAAGCCCACGATTTAG`
TIMP-2 −418 G/C	rs 8179090	RFLP-PCR	Forward	`CGTCTCTTGTTGGCTGGTCA`
TIMP-2 −418 G/C	rs 8179090	RFLP-PCR	Reverse	`CCTTCAGCTCGACTCTGGAG`
TIMP-2 303 C/T	rs 2277698	RFLP-PCR	Forward	`TAGGAACAGCCCCACTTCTG`
TIMP-2 303 C/T	rs 2277698	RFLP-PCR	Reverse	`CCTCCTCGGCAGTGTGTG`
MMP-1 −1607 1G/2G	rs 11292517	Fragment analysis	Forward	`6-fam-CCTCTGATGCCTCTGAGAAGA`
MMP-1 −1607 1G/2G	rs 11292517	Fragment analysis	Reverse	`TCCTCCCCTTATGGATTCCT`
MMP-2 −735 G/C	rs 2285053	TaqMan Allelic discrimination	Forward	`GGGTAAACCTCCCCACATTG`
MMP-2 −735 G/C	rs 2285053	TaqMan Allelic discrimination	Reverse	`ACAGTGGAAGGTCCCAGGTT`
MMP-2 −1306 C/T	rs 243865	ARMS-PCR	Forward	`TGTTGGGAACGCCTGACTTCAG`
MMP-2 −1306 C/T	rs 243865	ARMS-PCR	Reverse	`CTGACCCCCAGTCCTATCTGCC`
MMP-3 −1612 5A/6A	rs 35068180	Fragment analysis	Forward	`(6-Fam)CCTGCCTCAACCTCTCAAAG`
MMP-3 −1612 5A/6A	rs 35068180	Fragment analysis	Reverse	`AATTCACATCACTGCCACCA`
MMP-9 −1562 C/T	rs 34016235	Direct sequencing	Forward	`CATAGCTGGAAAATGGCAGA`
MMP-9 −1562 C/T	rs 34016235	Direct sequencing	Reverse	`TCGGGCAGGGTCTATATTCA`

ARMS, amplification refractory mutation system; RFLP, restriction fragment length polymorphism.