Figure 1.
Genetic deletion of MIF is protective against EAE. MIF-deficient (■) and wild-type (○) male C57Bl/6 mice were immunized with MOG35–55 and adjuvant on d 0. Clinical scores for each animal were monitored daily, and means were reported for each group. A) MIF-knockout mice were protected from EAE relative to wild-type controls. B–E) H&E staining of spinal cord (B, C) and brain sections (D, E) showed less perivascular infiltration in MIF−/− mice (C, E) vs. wild-type animals (B, D). F, G) Luxol fast blue with silver contrast showed less axonal severing in knockout mice (G) vs. wild-type (F). H) Quantification is representative of 4 sections of the brain and spinal cord (B–E). Results are representative of 3 separate experiments. *P < 0.05.