Table 2.

Mg²⁺-dependent mdADP release in wt and Y439A-m5aS1

	Rate constant (s−1)
Genotype	1 mM EDTA	5 mM Mg2+
wt (− actin)	43 ± 15	2.5 ± 0.3
wt (+ actin)	160 ± 20	23 ± 4
Y439A (− actin)	55 ± 20	2.7 ± 0.3
Y439A (+ actin)	170 ± 20	3.3 ± 0.6

mdADP release rate constants were determined as shown in Fig. 5B. Means ± se for n = 3–5 are listed. Conditions were as in Table 1, except that 1 mM EDTA was used instead of 5 mM MgCl₂ where indicated.