Figure 6. sLRP1 induces cytokine expression.

(A) RAW 264.7 cells were cultured in SFM for 1 h and then stimulated with sLRP1 (50 nM), vehicle, or as a control, sLRP1 (50 nM), which had been boiled. Cytokine expression was determined by qPCR. Results are expressed as the fold increase relative to the vehicle control. (B) RAW 264.7 cells were treated with mouse LRP1 or vehicle. Cytokine expression was determined. (C) Mouse LRP1 was pre-extracted with RAP-Sepharose. The extracted preparation and LRP1, which was not pre-extracted, were added to cultures for 5 h. Cytokine expression was determined. All expression studies were completed by qPCR (mean±sem; n=3). Relative expression of each cytokine was determined by comparison with cells that were treated with vehicle (*P<0.05).