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. 2010 Oct;88(4):715–724. doi: 10.1189/jlb.0410186

Figure 6. (A and B) Isoproterenol-induced increase in BMØ E. coli phagocytosis is mediated through β2-adrenergic receptors.

Figure 6.

(A) Percent PI of BMØ stimulated with 1 μM norepinephrine, epinephrine, or isoproterenol for 18 h. (B) Percent PI of BMØ pretreated with a selective β2-adrenergic antagonist ICI118551 (ICI) compound 30 min before the addition of isoproterenol. (C and D) Elicited PMØ response to isoproterenol stimulation. Sodium caseinate-elicited PMØ, obtained by Ficol density gradient, were stimulated with isoproterenol (1 μM) or vehicle overnight and stained with antibodies for anti-CD14-APC and anti-F4/80-FITC or were subjected to E. coli phagocytosis, stained with F4/80-PE, and analyzed by flow cytometry. (C) Representative histogram of CD14 expression in F4/80+ PMØ with MFI on the x-axis and percentage of cells on the y-axis. The area under the gray line is the isotype control. (D) Bar graph represents the PI (mean±sem) of PMØ and compared with BMØ (from B). Data are given as mean ± sem. *P < 0.05 versus vehicle; n = 4.