Figure 3.

Oligonucleotide-induced Type I/II message has an altered stability. (A) Cells were treated with 200 nM 106344 then E-selectin expression was stimulated by the addition of TNF at 5 ng/ml. After 2 h, TNF was removed and replaced with fresh media. Cells were harvested at various time-points following TNF removal, RNA isolated, then expression of E-selectin and G3PDH analyzed by northern blot. (B) Type III and Type I/II E-selectin RNA levels were normalized to G3PDH expression then plotted relative to the initial E-selectin level following TNF removal. Triangles, Type III; circles, Type I/II.