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. 2001 Mar 15;29(6):1293–1299. doi: 10.1093/nar/29.6.1293

Figure 5.

Figure 5

Anchored RT–PCR of E-selectin 3′-ends from antisense-treated HUVEC. (A) Ethidium bromide staining of 1.8% agarose gel containing the products of RT–PCR analysis. Oligonucleotides were administered for 4 h at 200 nM for single oligonucleotide treatment or 150 nM each for dual oligonucleotide treatment. Treated cells were incubated overnight prior to TNF induction of the E-selectin message: –, no TNF treatment; +, 5 ng/ml TNF for 2 h. Bold arrows, expected positions of the Types I, II and III transcripts; thin arrow, expected length of product for the variant poly(A) signal. (B) Diagrammatic representation of the oligonucleotide target sites and position of the PCR primers.