Figure 6.

Colocalization of viral transgene expression with spleen cell markers after treatment with clodronate liposomes. Frozen spleen sections (6 μm) were stained with antibodies for SIGNR1, CD11b, CD11c, B220, ER-TR7, and MAdCAM-1 in combination with an antibody to viral transgene, β-galactosidase (Table 1). Single stained and merged images are presented. An enlarged portion of each merged image is displayed in the far right panel, highlighted by a white box. White arrows indicate regions of colocalization (in yellow). WP indicates White pulp; MZ, Marginal zone, and RP, Red pulp. Images are representative of multiple fields of view from 2-4 different animals.