Table 1.
Antibodies used for IHC-Fr
| Antibody specificity | Isotype | Fixation | Clone | Final conc. | Source | Secondary antibody |
|---|---|---|---|---|---|---|
| β-galactosidase (Escherichia coli) | rabbit IgG1 | Acetone/4% PFA | 8.3 μg/mL | MP Biomedical | Gt α-Rb IgG Alexa488 | |
| B220 (CD45R) | rat IgG2a, κ | 4% PFA | RA3–6B2 | 1.25 μg/mL | eBiosciences | Gt α-Rt IgG Alexa488/546 |
| CD11b | rat IgG2b, κ | Acetone | M1/70 | 0. 5 μg/mL | Abcam | Gt α-Rt IgG Alexa488/546 |
| CD11c (αXβ2 integrin) | Ar hamster IgG | Acetone | N418 | 2.5 μg/mL | Abcam | Gt α-Ham IgG Alexa488/546 |
| CD169 (Sialoadhesin) | rat IgG2a | 4% PFA | Moma-1 | 0. 5 μg/mL | Bachem | Gt α-Rt IgG Alexa488 |
| CD209b (SIGNR1) | rat IgM | 4% PFA | ER-TR9 | 4 μg/mL | Abcam | Gt α-Rt IgM Alexa488/594 |
| F4/80 (MΦ) | rat IgG2a | 4% PFA | CI:A3–1 | 20 μg/mL | Abcam | Gt α-Rt IgG Alexa488 |
| MAdCAM-1 (mucosal vascular addressin-1) | rat IgG2a, κ | Acetone | MECA-367 | 10 μg/mL | eBiosciences | Gt α-Rt IgG Alexa488/546 |
| MARCO (SR-A) | rat IgG1 | 4% PFA | ED31 | 2 μg/mL | Bachem | Gt α-Rt IgG Alexa488 |
| Reticular fibroblast and fibres Ab | rat IgG2a | 4% PFA | ER-TR7 | 1 μg/mL | Abcam | Gt α-Rt IgG Alexa488 |
Antibodies used in this study are listed alphabetically. Isotype controls were as follows: rabbit IgG1 (Vector Laboratories); rat IgG2a (Abcam); rat IgG2b (Abcam); Armenian hamster IgG (eBiosciences); rat IgM (Abcam); and rat IgG1 (Vector Laboratories).
IHC-Fr indicates immunohistochemistry frozen; CD, cluster of differentiation molecule; MΦ, macrophage; SIGNR1, specific ICAM3 grabbing nonintegrin related-1; SR-A; scavenging receptor A; Ab, antibody; IgG, immunoglobulin G; Ar Ham, Armenian hamster; IgM, immunoglobulin M; PFA, paraformaldehyde; Gt, goat; Rb, rabbit; and Rt, rat.