Table 1.
Hif-2 regulates iron metabolism gene expression in hepatocytes
| Gene | VHL | VHLHif1 | VHLHif2 | VHLHif1Hif2 |
|---|---|---|---|---|
| Epo | 1024.63* | 1046.88* | 23.23* | 2.43 |
| Vegf | 7.97* | 7.33* | 1.29 | 0.91 |
| Cp | 3.58* | 2.47* | 0.67* | 0.98 |
| Heph | 5.76* | 5.20* | 1.16 | 0.91 |
| Aco1 | 0.83 | 1.12 | 0.99 | 0.91 |
| Ireb2 | 0.95 | 0.86 | 0.99 | 1.08 |
| Alas2 | 2.08* | 10.33* | 1.2 | 0.89 |
| Hamp | 0.58* | 0.27* | 0.21* | 1.25 |
| Hfe | 2.28* | 2.15* | 1.55* | 1.36* |
| Hfe2 | 0.61* | 0.84 | 0.97 | 1.56 |
| Dmt1 | 10.52* | 17.22* | 0.88 | 1 |
| Mfrn | 4.46* | 7.42* | 0.8 | 0.99 |
| Fpn | 3.12* | 1.84 | 0.92 | 0.87 |
| Trf | 3.25* | 2.39* | 0.89 | 0.93 |
| Trfc | 8.37* | 4.01* | 1.7 | 0.81 |
| Trfr2 | 0.62* | 0.88 | 1.02 | 0.95 |
| Lcn2 | 74.84* | 49.26* | 0.71 | 1.06 |
Shown are fold changes in mRNA expression levels of genes regulating iron metabolism. Analyzed were Albumin-Cre conditional mutant mice and compared with Cre-negative littermates. First column, pVHL-deficient livers (VHL) from Albumin-Cre/Vhlh mutant mice compared with Cre-negative littermate controls (n = 4-8 each); second column, livers that lack pVHL and Hif-1α and express high levels of Hif-2α (VHLHif1) compared with Cre-negative littermates (n = 4-8 each); third column, livers that lack pVHL and Hif-2α but express high levels of Hif-1α (VHLHif2) compared with Cre-negative littermates (n = 5-8 for mutants and n = 5 for Cre-negative littermates); fourth column, livers that lack pVHL, Hif-1α, and Hif-2α (VHLHif1Hif2) compared with Cre-negative littermates (n = 4-8 each).
Vegf indicates vascular endothelial growth factor; Cp, ceruloplasmin; Heph, hephaestin; Aco1, aconitase; Ireb2, iron regulatory protein 2; Alas2, δ-aminolevulinate synthase 2; Hamp, hepcidin; Hfe, hemochromatosis protein.
P < .05.