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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Traffic. 2010 May;11(5):660–674. doi: 10.1111/j.1600-0854.2010.1045.x

Figure 8.

Figure 8

Reduced NF-κB and CREB activation can be restored by overexpression of rGASP-1. A,B) US28-mediated transcription factor activation is rescued upon overexpression of rGASP-1. ShScr infected cells (black bars) and shGASP-1 infected cells (grey and striped bars) were transfected with Flag-US28 (50 ng/well) in combination with 100 ng/well of pcDNA3.1 (black bar and grey bars) or HA-rGASP-1 (striped bars) as well as the respective cis-reporter plasmids for either NF-κB (50 ng/well, A) or CREB (200 ng/well, B). Controls were transfected with pcDNA3.1 (white bars). A luciferase reporter assay was conducted 24 h post-transfection. Values were normalized to the cell number, whereby 100% corresponds to the basal activity of US28 (black bar). Data are means of four experiments ± SD carried out in quadruplicates, ***p < 0.001. C,D) US28 levels in shGASP-1 infected and rGASP-1 transfected HEK 293 cells. HEK293 cells were infected and transiently transfected as described in A and B. The expression levels of US28 were assayed by ELISA as described before.