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. Author manuscript; available in PMC: 2010 Nov 5.
Published in final edited form as: Biosci Rep. 2009 Dec 15;30(3):159–168. doi: 10.1042/BSR20090008

Figure 3.

Figure 3

ARHI can block pStat3β nuclear translocation. (A) Stat3-importin binding analyzed by GST-pull down assays. SKBr3 cells were treated with or without IL-6 (10 ng/mL) for 30 minutes before cells were harvested for cell lysate preparation. Ten GST-importin (Imp) fusion proteins and Stat3/pStat3 antibodies were used to detect binding between Stat3 and importins. GST protein was used as a control. Loading was measured with anti-GST antibody. (B) Nuclear import assay for pStat3β-GFP. Nuclear import assays were performed as described in Materials and Methods. Purified pStat3β and RanGDP, importin α7, and importin β1 were incubated with permeabilized HeLa cells. Import assays were also performed by adding ARHI protein.