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. 2010 Nov 5;5(11):e15420. doi: 10.1371/journal.pone.0015420

Figure 6. Increased LKB1 Serine 307 phosphorylation by Berberine in BAEC.

Figure 6

A. Inhibition of AMPK activity by ONOO−. Recombinant AMPKα1β1γ1 was treated with chemically synthetized ONOO- at concentrations indicated for 5 minutes and AMPK activity was assayed as described in Methods and Materials. N = 5 *P<0.05; control verse ONOO−-treated; B. Effects of okadaic acid (OA) on Berberine-enhanced phosphorylation of AMPK-Thr172 and ACC-Ser79 in BAEC. The blot is representative of three blots. from three individual experiments. C. Effects of transfection of PP2A siRNA on Berberine-enhanced phosphorylation of AMPK-Thr172 and ACC-Ser79 in BAEC. The blot is representative of three blots from three individual experiments. D. Effects of STO-609 on Berberine-enhanced phosphorylation of AMPK-Thr172 and ACC-Ser79 in BAEC. The blot is representative of three blots. E. Effects of berberine on the phosphorylation of LKB1 serine 307 in BAEC. The blot is representative of three blots from three individual experiments. F. Genetic inhibition of LKB1 abolishes Berberine-induced phosphorylation of AMPK-Thr172 in BAEC. The blot is representative of three blots from three individual experiments. G Effects of Berberine on AMPK phosphorylation at Thr172 in LKB1-deficient A549 cells.