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. 2010 Nov 5;5(11):e13828. doi: 10.1371/journal.pone.0013828

Figure 1. Structure of versican G3 domain and variable versican expression in mouse mammary tumor cells.

Figure 1

(a) Immunoblotting showed that 4T1 cells expressed highest level of versican V1 isoform, vimentin, pERK; 67NR and 66c14 cells expressed N-cadherin, while 4T07 and 4T1 cells expressed E-cadherin; In 20 ng/ml EGF medium, 4T1 cells expressed increased pEGFR and pERK. EGFR*: adding 20 ng/ml EGF for 5 min; ERK*: adding 20 ng/ml EGF for 60 minutes. (b) Versican G3 construct (upper) was expressed in 66c14 cells, analyzed by western blot using cell lysate and culture medium (lower). (c) Morphologically, the G3-transfected 66c14 cells appeared more elongated and spreading more evenly as compared with the predominantly cuboid appearance of cells in the control group that tended to aggregate into clusters.