Figure 10. Versican G3 domain modulates breast cancer cell growth and migration via its epidermal growth factor-like motifs.

(a) Vector-, G3- and G3ΔEGF- transiently transfected 66c14 cells and 4T07 (2×10⁴) were inoculated in 6-well culture dishes containing 10% FBS/DMEM and cultured for 3 days. Cells were counted under light microscopy. (b) Vector-, G3- and G3ΔEGF- transiently transfected 66c14 cells (2×10⁵) were inoculated and cultured in 6-well culture dishes containing 10% FBS/DMEM for 12 h. all the samples were wounded by a sterile pipette tip to create a 1-mm cell-free path, washed with PBS, cultured in 10% FBS/DMEM medium with 2 mM hydroxyurea for 3 d. (c) The distances between the wounding centre and the front of the migrating cells (vertical axis) were measured for statistical analysis. (All groups compared with vector control cells, n = 10, * p<0.05, ** p<0.01, analyzed with t-test). (d) Cell lysates of vector-, G3- and G3ΔEGF- transiently transfected 66c14 cells were prepared and subjected to immunoblotting with antibodies to 4B6, pEGFR, EGFR, pERK and ERK2.