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. 2010 Nov 5;5(11):e13864. doi: 10.1371/journal.pone.0013864

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Manetti et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunoblot analysis of cell surface extracts of GAS strains 27_M6 (FCT-1), 51_M1 (FCT-2), 43_M3 (FCT-3), 135_M12 (FCT-4) 57_M28 (FCT-4) and 96_M89 (FCT-4) grown in non-buffered C-medium at starting pH values of 7.5 or 6.4 up to OD₆₀₀ of 0.4; nitrocellulose transferred extracts were incubated with specific mouse polyclonal sera raised against M protein variants,Spy0269 protein, specific AP-1 pilin proteins and, in the case of the non biofilm formers M28 and M89, specific AP-1 and BP proteins.