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. 2010 Jun 18;118(11):1597–1602. doi: 10.1289/ehp.1002153

Figure 2.

Figure 2

DNA methylation status of p16INK4a, p15INK4b, Myc, Bid3, and Sfn promoter region in liver samples from control rats (C1, C2) or rats treated with furan (2 mg/kg bw for 4 weeks; T1, T2) (n = 5 rats). MSP profiles of p16INK4a and Myc promoter region (A) and p15INK4b promoter (B). COBRA results of c-myc gene digested by TaqI 241 bp (40/77/125) (C), Bid3 gene digested by BstUI 310 bp (210/68/32) (D), and Sfn gene digested by TaqI 229 bp (56/173) (E). Abbreviations: bp, base pairs; C, cleaved PCR products; M100, 100-bp DNA ladder (New England Biolabs); Me, methylated rat genomic DNA treated with methyltransferase (M.SssI); MV, HyperLadder V (Bioline, Luckenwalde, Germany); UM, uncleaved PCR products.