Effect of HK and HHK branching
polymers on transfection efficiency of liposome. (A)
Comparison of HK with branched polymers. Four polymers (HK, HK2b,
HK3b and HK4b) at various concentrations (0.06, 0.18, 0.375, 0.75,
1.5, 3.0 and 7.5 nmol) with liposomes were examined for their ability
to increase luciferase expression in MDA-MB-435 cells. The polymer–liposome–plasmid
DNA complexes were prepared as described in Materials and Methods.
The dashed lines represent the polymer dosage toxic to cells. (B) Five polymers (HK, HHK, HHK2b, HHK3b and HHK4b)
were compared at various concentrations as described in (A).