Branched polymer enhances
transfection of several liposome preparations. HK (7.5 nmol) or
HHK4b (0.375 nmol) co-polymer was initially mixed with 0.75 µg of DNA (PCI-luc) for 30 min, various
liposome preparations were then added for an additional 30 min.
Forty-eight hours later luciferase activity was measured. The following
liposome amounts were added: DOTAP, 1.5 µg;
lipofectin, 1 µg; lipofectamine, 5 µg; and DOSPER, 1.5 µg. *, P < 0.05, HK versus no polymer with all
liposome preparations; **, P < 0.05,
HHK4b versus HK with all liposome preparations.