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Branched polymers increase the transfection efficiency in the presence of serum. The optimal dosage of HK (7.5 nmol), HK4b (0.375 nmol) or HHK4b (0.75 nmol) was mixed with 0.75 µg of PCI-luc and 1.5 µg of liposome. The complex was added to the cells for a 4 h incubation in the presence of 10% FCS. *, P < 0.05, HK4b versus HK or HHK4b versus HK. Anova on Ranks with Dunnet’s multiple comparison.
