Table 1.
The primer sequences and annealing temperatures for the seven genes studied
| Gene | Annealing temperature(°C) | Primer sequence | Amplicon (bp) | Accession No. | |
|---|---|---|---|---|---|
| β-actin | 60 | Forward Reverse |
ATCGTGCGTGACATTAAGGAGAAG AGGAAGGAAGGCTGGAAGAGTG |
179 | NM_001101 |
| β1-AR | 60 | Forward Reverse |
GGGAGAAGCATTAGGAGGG CAAGGAAAGCAAGGTGGG |
270 | NM_000684 |
| β2-AR | 60 | Forward Reverse |
CAGCAAAGGGACGAGGTG AAGTAATGGCAAAGTAGCG |
334 | NM_000024 |
| COX-2 | 57 | Forward Reverse |
TTGACCAGAGCAGGCAGATG CCAGAAGGGCAGGATACAGC |
171 | NM_000963.2 |
| VEGF-A | 57 | Forward Reverse |
CTGGGCTGTTCTCGCTTCG CTCTCCTCTTCCTTCTCTTCTTCC |
140 | NM_001025370.1 |
| EGFR | 53 | Forward Reverse |
AGG ACA GCA TAG ACG ACA C AGG ATT CTG CAC AGA GCC A |
90 | NM_005228.3 |