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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Plant J. 2010 Sep;63(6):889–900. doi: 10.1111/j.1365-313X.2010.04295.x

Figure 3. Quantification of receptor interactions.

Figure 3

(a) Solubilized membrane extracts from CLV1-GFP co-expressed with BAM2-FLAG in transient expression were IPd with anti-GFP antibodies and co-IPs were detected with anti-FLAG antibodies. A dilution series of 10%, 5% and 1% of total bound and unbound fractions were assayed on protein gel blots to estimate the efficiency of IP and co-IP (see Experimental Procedures).

(b) CRN-GFP co-expressed with CLV2-MYC in transient expression was IPd with anti-GFP antibodies and co-IPs were detected with anti-MYC antibodies. A dilution series of 2%, 1% and 0.2% of total bound and unbound fractions were assayed on protein gel blots to estimate the efficiency of IP and co-IP.

(c) CLV1-FLAG co-expressed with CLV2-MYC in transient expression was IPd with anti-FLAG antibodies and co-IPs were detected with anti-MYC antibodies. A dilution series of 8%, 4%, 1%, 0.4% and 0.1% of total bound, and a dilution series of 4%, 1%, 0.4%, 0.1%, and 0.04% of unbound fractions were assayed on protein gel blots to estimate the efficiency of IP and co-IP.

(d) CLV1-FLAG co-expressed with CLV2-MYC and CRN-GFP in transient expression was IPd with anti-FLAG antibodies and co-IPs were detected with anti-MYC. A dilution series of 8%, 4%, 1% and 0.4% of total bound, and a dilution series of 4%, 1%, 0.4% and 0.1% of unbound fractions were assayed on protein gel blots to estimate the efficiency of IP and co-IP.