Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2011 Sep 1.

Published in final edited form as: Photochem Photobiol. 2010 Sep-Oct;86(5):1099–1108. doi: 10.1111/j.1751-1097.2010.00780.x

Aggregation of viral proteins is induced by reactive oxygen species. (a) The addition of glutathione (20 mM) prior to UV irradiation significantly reduced the higher molecular weight aggregates, as can be seen in western blots probed for p24 and gp41. (b) Oxygen depletion prior to UV irradiation for 15 minutes in the presence of the azido compounds, significantly reduced the aggregation at higher molecular weights, while maintaining viral inactivation. Lane A is the HIV control (No UV), the remaining lanes are HIV + compound listed + UV irradiation for 15 minutes. B: DMSO, E: AZNAP, F:DAN, G: INA, H: DABIPH.

Aggregation of viral proteins is induced by reactive oxygen species. (a) The addition of glutathione (20 mM) prior to UV irradiation significantly reduced the higher molecular weight aggregates, as can be seen in western blots probed for p24 and gp41. (b) Oxygen depletion prior to UV irradiation for 15 minutes in the presence of the azido compounds, significantly reduced the aggregation at higher molecular weights, while maintaining viral inactivation. Lane A is the HIV control (No UV), the remaining lanes are HIV + compound listed + UV irradiation for 15 minutes. B: DMSO, E: AZNAP, F:DAN, G: INA, H: DABIPH.