Skip to main content
. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Pharmacol Res. 2010 Sep 8;62(6):500–505. doi: 10.1016/j.phrs.2010.08.003

Figure 2.

Figure 2

Effects of various inhibitors on agonist-induced intracellular calcium mobilization in RBL-2H3 cells. A. Effects of pretreatment with PTX, a phospholipase C inhibitor U73122 and various concentrations of the P2Y1 receptor antagonist MRS2500. Cells were pretreated with PTX (100 ng/ml) for 24 h or with U73122 (10 μM) and MRS2500 for 20 min before the measurements. B. Schild analysis of antagonism by MRS2500 of the agonist effect of ADP. C. Effect of MRS2500 on the selective P2Y1 receptor agonist MRS2365-induced calcium release. D. Schild analysis of antagonism by MRS2500 of the agonist effect of MRS2365. The calcium mobilization was measured 20 s after addition of agonists and lasted for 120 s. The protocol used for measurement of calcium mobilization was similar to previously described [29]. The EC50 values (nM) of ADP and MRS2365 were 278 ± 42 and 4.5 ± 0.8 nM, respectively.