Figure 5.
A. Effects of P2Y receptor antagonists on ADP-induced enhancement of antigen-mediated Hex release in RBL-2H3 cells. MRS2500 and MRS2211 are antagonists for the P2Y1 and P2Y13 receptors, respectively. Cells were primed with anti-DNP-BSA antibody for 24 h before testing for the release of Hex. Results are expressed as mean ± S.E. and were from 3-4 separate experiments performed in duplicate. B. Effect of P2Y1 receptor-specific siRNA on ADP-induced enhancement of antigen-induced Hex release. The experimental procedures were as described previously [6].