Table 2.
Effect of 10 µmol/L Compound 2 on vasomotor responses to 5-HT, S1P and PE in basilar and femoral arteries of rat: pharmacological parameters.
| pD2 | EC50 (µmol/L) | Emax (% K+) | |
|---|---|---|---|
| 5-HT | |||
| Basilar | |||
| Control | 7.01 ± 0.12 | 0.10 (0.06–0.17) | 110.1 ± 5.2 |
| Compound 2 | 6.24 ± 0.29 | 0.57 (0.16–2.10) | 34.4 ± 5.3** |
| Femoral | |||
| Control | 6.69 ± 0.09 | 0.20 (0.14–0.30) | 129.4 ± 4.9 |
| Compound 2 | 6.42 ± 0.09 | 0.38 (0.25–0.57) | 117.4 ± 5.2 |
| S1P | |||
| Basilar | |||
| Control | 5.62 ± 0.19 | 2.41 (0.99–5.89) | 62.2 ± 6.2 |
| Compound 2 | 5.09 ± 0.19 | 8.14 (3.42–19.4) | 52.5 ± 7.4 |
| PE | |||
| Femoral | |||
| Control | 5.36 ± 0.09 | 4.36 (2.86–6.64) | 94.6 ± 4.7 |
| Compound 2 | 5.66 ± 0.08 | 2.21 (1.50–3.24) | 89.7 ± 3.5 |
Pharmacological parameters are from non linear regression; pD2 is the negative logarithm of the concentration producing 50% of the maximum effect, EC50 is the concentration producing 50% of the maximum effect (95% confidence limits are given in parenthesis), Emax is the maximum effect (in % of vasoconstriction evoked in the same preparations by 100 mmol/L K+).
Data are mean ± SEM of 5–12 independent measurements. Statistical comparisons were made on the whole curves by two-way ANOVA;
**
P<0.01 versus Control.