Table 4.
Pharmacological parameters of vasomotor responses to U46619, PE and S1P in arteries of WT, SPK1−/− and SPK2−/− mice.
| pD2 | EC50 (µmol/L) | Emax (mN/mm) | |
|---|---|---|---|
| U46619 | |||
| Basilar | |||
| WT | 7.64 ± 0.25 | 0.02 (0.01–0.07) | 0.74 ± 0.06 |
| SPK1−/− | 7.80 ± 0.38 | 0.02 (0.01–0.09) | 0.42 ± 0.05** |
| SPK2−/− | 7.27 ± 0.30 | 0.05 (0.01–0.21) | 0.76 ± 0.08 |
| PE | |||
| Mesenteric | |||
| WT | 6.06 ± 0.16 | 0.87 (0.41–1.84) | 1.56 ± 0.10 |
| SPK1−/− | 6.13 ± 0.26 | 0.75 (0.22–2.53) | 1.69 ± 0.16 |
| SPK2−/− | 6.05 ± 0.17 | 0.90 (0.41–1.96) | 1.65 ± 0.11 |
| S1P | |||
| Basilar | |||
| WT | 6.25 ± 0.25 | 0.56 (0.18–1.78) | 0.60 ± 0.05 |
| SPK1−/− | 6.44 ± 0.50 | 0.36 (0.04–3.63) | 0.39 ± 0.06* |
| SPK2−/− | 5.78 ± 0.29 | 1.65 (0.43–6.36) | 0.62 ± 0.07 |
| Mesenteric | |||
| WT | 6.32 ± 0.25 | 0.47 (0.15–1.47) | 1.29 ± 0.11 |
| SPK1−/− | 6.45 ± 0.46 | 0.36 (0.04–2.99) | 1.37 ± 0.19 |
| SPK2−/− | 5.58 ± 0.18* | 2.69 (1.18–6.14) | 1.48 ± 0.14 |
Pharmacological parameters are from non linear regression; pD2 is the negative logarithm of the concentration producing 50% of the maximum effect, EC50 is the concentration producing 50% of the maximum effect (95% confidence limits are given in parenthesis), Emax is the maximum effect.
Data are mean ± SEM of 5–12 independent measurements. Statistical comparisons were made on the whole curves by two-way ANOVA;
*
P<0.05
**
P<0.01 versus Control.