Skip to main content
. 2001 Mar 15;29(6):1317–1325. doi: 10.1093/nar/29.6.1317

Figure 2.

Figure 2

hMre11 binds to ssDNA and dsDNA molecules. (A) Radiolabeled 50 nt ssDNA or blunt-ended dsDNA (MJ19 and MJ19/20, respectively) were incubated at 1.25 nM with the indicated amounts of hMre11 under standard conditions in the absence of divalent cations. Samples were analyzed by native PAGE, followed by autoradiography. nb, non-bound probe; b, bound probe; DNA–protein complex. (B) Biotinylated 50 nt ssDNA or blunt-ended dsDNA (MJ29 and MJ29/20, respectively) were coupled to streptavidin-coated magnetic beads and incubated at 20 nM with 24 nM hMre11 preparation under standard conditions supplemented with 0.1% NP-40. Bound fractions were analyzed by SDS–PAGE and immunoblotting with affinity-purified α-hMre11 antibodies. Lane 1, size marker (m, molecular mass indicated in kDa); lane 2, incubation with control beads lacking DNA; lane 3, incubation with beads coupled to ssDNA; lane 4, incubation with beads coupled to dsDNA.