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. 2001 Mar 15;29(6):1317–1325. doi: 10.1093/nar/29.6.1317

Figure 4.

Figure 4

hMre11 anneals complementary ssDNA molecules. Two complementary 50 nt ssDNAs (MJ19 and MJ20), one of which was radiolabeled, were incubated with 10 nM hMre11, hKu70/80, hRad52 or without protein. After deproteinization, samples were analyzed by native PAGE and autoradiography. Lane 1, incubation without protein; lane 2, dsDNA, annealed by heating and slow cooling; lane 3, incubation with hMre11; lane 4, incubation with hKu70/80 heterodimer; lane 5, incubation with hRad52. The positions of ssDNA and dsDNA are indicated.