RNA template was isolated at 8 hpi from rifampicin-treated (+Rif) and mock-treated (−Rif) cells. L, 100 bp DNA ladder (size designated on left). +RT/−Rif, with reverse transcriptase and mock-treated. −RT/−Rif, without reverse transcriptase and mock-treated. +RT/+Rif, with reverse transcriptase and rifampin-treated. −RT/+Rif, without reverse transcriptase and rifampin-treated. C, DNA control.