TABLE 1.

Oligonucleotide primers used in this study.

Gene Target	RefSeq ID	Designation	Gene Primer Sequence (5’ → 3’)	Purpose
icmT	CBU 1641	CB62f	GCAAAATCGCCATAGCATGGTG	RT-PCR
		CB578f	GGGATGGCAAAACAGCCTTT	RT-qPCR
		CB579r	CCGTCACCGCTACGATGAG	RT-qPCR
		CB78f	†CACCATGAAATCTCTCGATGAGG	PCR, RT-PCR
		CB79r	‡TTAGTTATCCCACCATGCTATGG	PCR, RT-PCR
dotD	CBU 1643	CB63f	TCCAGACGGATCATTGAGC	RT-PCR
		CB61r	CAACGCCAGAAAGAGGGGCAGC	RT-PCR
dotB	CBU 1645	CB64r	CAATGTGTTTGGGTTCGAAGCG	RT-PCR
		CB603f	ACTCGACAGTGATCCCGAAC	RT-PCR
		CB586f	CCACGGGTTCGGGTAAAAG	RT-qPCR
		CB587r	GCGCTTCGGCCAATTCT	RT-qPCR
glycine betaine transporter§	CBU 1646	CB602r	TTACCCAGCGGCGTTAATAC	RT-PCR
hypothetical ORF	CBU 1647	CB716r	ACGCTAACGGCGTTAATACG	RT-PCR
dotA	CBU 1648	CB60r	CGATAATGCCTTCATTGAGC	RT-PCR
		CB718f	TAGTGGCAGTATCCTTCACG	RT-PCR
		CB654f	ACAATCAATCCCCGTTGAAA	RT-qPCR
		CB655r	AGCTATCATCGCCTGGCTTA	RT-qPCR
icmV	CBU 1649	CB59f	TTCCAAATGAAGCAACGC	RT-PCR
		CB592f	TGGCGGGTTACACGGTTT	RT-qPCR
		CB593r	CGCAGACGAAAGCCGATAA	RT-qPCR
		CB70f	†CACCATGATTCTTTTGGAGTCTTCC	RT-PCR
		CB71r	‡TTATTGTTTGGACCCCTTAAAGGTG	RT-PCR
icmW	CBU 1650	CB58f	CAAACTCTTGAGGAAGG	RT-PCR
		CB594f	CGCCGCTGCGAAAGTG	RT-qPCR
		CB595r	ACCGGCGGTGTCTATTTCC	RT-qPCR
		CB40f	†CACCATGCCAGATCTGTCGC	RT-PCR
		CB41r	‡TTATAAACCACCTTCCTCAAGAG	RT-PCR
icmX	CBU 1652	CB57r	GAAGCAATACCAAGAACACG	PCR, RT-PCR
		CB658f	CCGCTTATAATTCGGACCAA	RT-qPCR
		CB659r	TTGATAAGCGGGATTGTTCA	RT-qPCR

^†CACC, non-C. burnetii sequence, engineered directional cloning sequence for pET200/D-TOPO plasmid.

^‡TTA, non-C. burnetii sequence, engineered stop codon.

^§glycine betaine transporter (Beare, et al., 2009).

^{f, r}designate Forward and Reverse primers respectively.