Figure 5.

The effect of nitrogen limitation on the production of PNMA and PNMB. Wild-type A. nidulans mycelia were grown with glucose (0.4% w/v) as carbon source and ammonium tartrate or sodium nitrate as nitrogen sources at concentrations of 5 and 40 mM. Mycelia were harvested after 19 h growth and 1 μg of cell-free extract from each harvest was incubated with 2 μg NmrA as described in the Legend to Figure 3. Lane 1 = glucose plus 5 mM ammonium, Lane 2 = glucose plus 40 mM ammonium, Lane 3 = glucose plus 5 mM nitrate, lane 4 = glucose plus 40 mM nitrate, Lane 5 = nondigested NmrA control. The digestion products were separated by SDS-PAGE using a 10% acrylamide separating gel and the positions of 45 and 29 kDa markers are shown.