Tabel I.
The Measured and Predicted Mrs of NmrA and its Proteolytic Products
| Sample | Measured Mr (Da) after SDS-PAGE and gel extraction | Measured Mr (Da) prior to SDS-PAGE and gel extraction | Predicted Mr (Da) from the amino acid sequence | Difference (Da) from measured Mr of FNM1A | Difference (Da) from measured Mr of FNM1B |
|---|---|---|---|---|---|
| FNM1A | 33,402.2 | NA | — | — | — |
| FNM1B | 32,134.6 | 32,059.7 | 32059.8 | — | — |
| NmrA 2–352 | 38,737.5 | 38,663.8 | 38,662.6 | — | — |
| NmrA 2–291 | NA | NA | 32,059.7 | — | −74.9 Da |
| NmrA 2–292 | NA | NA | 32,116.8 | — | −17.8 Da |
| NmrA 2–293 | NA | NA | 32,230.0 | — | +95.4 Da |
| NmrA 2–306 | NA | NA | 33,330.2 | −74.0 Da | — |
| NmrA 2–307 | NA | NA | 33,458.4 | +54.2 Da | — |
The measured Mrs of NmrA lacking the N-terminal methionine (NmrA 2–352) and the FNM1B proteolytic fragment determined before and after SDS-PAGE and gel elution are shown with the predicted Mr derived from the amino acid sequence. A difference of +74.9 Da is evident in each case. Also shown are the measured Mrs of the NmrA proteolytic products FNM1A and FNM1B after gel extraction along with the predicted Mrs of C-terminally deleted NmrA possible candidate sequences.