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. 2010 Sep 16;21(10):1287–1297. doi: 10.1089/hum.2010.049

FIG. 1.

FIG. 1.

Time course of ϕC31 integrase protein expression within mouse liver. Twenty micrograms each of pCSI-HA and pVFB were hydrodynamically injected into the tail vein of mice. At various time points over a 6-month time course, the mice were killed, their livers were removed, and total protein and DNA were isolated. (A) Western blot analysis of total protein isolated from mouse liver showed the presence of ϕC31 integrase at 3 hr postinjection. Integrase protein levels increased up to a peak at 16 hr and were undetectable by 24 hr. Below each lane of the blot is the percentage of total ϕC31 integrase protein present, as quantified by densitometry. (B) Immunofluorescence staining for ϕC31 integrase in mouse liver sections obtained over a time course. The top row shows DAPI-stained nuclei, whereas the bottom row shows staining for ϕC31 integrase. Numbers inside the images correspond to the percentage of cells that are expressing ϕC31 integrase protein. Color images available online at www.liebertonline.com/hum.