FIG. 2.

Integration of donor plasmid occurs rapidly after plasmid injection. DNA extracted from liver was subjected to PCR using primers that detect the junction between the attB site of the donor pVFB plasmid and mpsL1, a commonly used pseudo-attP site in the mouse genome. Lane 1, molecular weight markers; lane 2, no DNA; lane 3, liver DNA sample known to have integration at mpsL1; lane 4, liver that received saline alone; lanes 5–25, time course of DNA samples from mice receiving pVFB and pCSI-HA, after the specified time in hours (1–48 hr), followed by days (3–180 days).