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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Neurosci Methods. 2010 Aug 15;191(1):1–10. doi: 10.1016/j.jneumeth.2010.05.017

Figure 4. Rapid Uptake and Diffusion of Dye.

Figure 4

A) Schematic illustrating the experimental design to observe and evaluate loading at various time intervals in the main olfactory bulb. The mitral cell layer was imaged after electroporating a single glomerulus. B) Graph of the ratio of pixel intensities measured at the soma divided by the background plotted against time after completion of the electroporation protocol. C-D) Fluorescent images of the mitral cell layer taken at 5, 10, and 20 minutes after electroporation of a glomerulus. Inset) The difference in pixel intensities between the cell body (black bar), and the surrounding tissue (yellow bar) was calculated for each time point.