Table 1.
Comparison of Dyes Used
Dextran Dyes | Concentration | Vortexed | Sonicated | Centrifuged |
---|---|---|---|---|
Rhodamine Ruby Red | 12% in PBS |
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Oregon Green Bapta | 5% in PBS |
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|
Alexa Fluor 594 | 7% in PBS |
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Hydrazide Dyes | Concentration | Vortexed | Sonicated | Centrifuged |
---|---|---|---|---|
Alexa Fluor 350 | 1mM in IBS |
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Alexa Fluor 488 | 1mM in IBS |
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Alexa Fluor 594 | 1mM in IBS |
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Alexa Fluor 647 | 1mM in IBS |
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